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richard youle  (Addgene inc)


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    Structured Review

    Addgene inc richard youle
    Richard Youle, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/richard+youle/pHAGE-mt-mKeima+(Plasmid+%23131626)/pmc12758248-381-14-16
    Average 95 stars, based on 50 article reviews
    richard youle - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Leveraging BRG1 Driven Ferroptosis Resistance to Overcome Treatment Resistance
    Article Snippet: .. Mitophagy was measured using mt-Keima ratiometric probe. pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid # 131626 ; http://n2t.net/addgene:131626 ; RRID:Addgene_131626). ..

    Article Title: LMNA R644C Associates With Hepatic Steatosis in a Large Cohort and Increases Cellular Lipid Droplet Accumulation in Vitro
    Article Snippet: .. The LMNA open reading frame was cloned into mCherry-Parkin, Addgene plasmid #23956 (replacing the Parkin open reading frame), via the BspEI and BamHI sites. mCherry-Parkin was a gift from Richard Youle (Addgene plasmid #23956; http://n2t.net/addgene:23956; RRID:Addgene 23956)15. .. The R644C variant of LMNA was generated using the QuikChange II site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and verified via Sanger sequencing.

    Article Title: Early tolerance and late persistence as alternative drug responses in cancer.
    Article Snippet: .. Cells were acquired at CytoFLEX LX (BeckmanCoulter) and analysedbyFlowJo (BectonDickinson v10.8.1). pHAGE-mt-mKeimawas a gift from Richard Youle (Addgene plasmid # 131626; http://n2t.net/ addgene:131626)57. ..

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression
    Article Snippet: siRNAs against BAX (#2: HSS141355 and #3: HSS141356, Thermo Fisher Scientific) and p53 (TP53, #2: HSS186390 and #3: HSS186391, Thermo Fisher Scientific) or AllStars Negative Control siRNA (QIAGEN, Venlo, The Netherlands) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. .. The plasmid hBax C3-EGFP, a gift from Richard Youle (pEGFP-C3-hBAX: Addgene plasmid #19741; https://www.addgene.org/19741/ (accessed on 11 August 2025); RRID:Addgene_19741; Addgene, Watertown, MA, USA) [ ], and the control plasmid pEGFP-C3 (GenBank accession # U57607 ; Takara Bio Inc., Shiga, Japan) were transiently transfected using Lipofectamine 2000 (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. ..

    Article Title: PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy
    Article Snippet: PPA2 polyclonal antibody (PA5–56816, 1:10000), V5 Tag polyclonal antibody (A190-119A), Alexa Fluor 568 (A-11004; anti-mouse, 1:500), Alexa Fluor 568 (A-11011; anti-rabbit, 1:500), Alexa Fluor 488 (A-11008; anti-rabbit, 1:500), and Alexa Fluor 488 (A-11001; anti-mouse, 1:500) were purchased from Invitrogen. .. PPA2 (HsCD00950939) plasmid was purchased from DNASU Plasmid Repository. pHAGE-mt-mKeima was a gift from Richard Youle (Addgene 131626), pLKO.1 - TRC cloning vector was a gift from David Root (Addgene 10878), psPAX2 was gifted by Didier Trono (Addgene 12260), pMD2.G was gifted by Didier Trono (Addgene 12259), pTFAM.1.0-gDNA was a gift from Kevin White (Addgene 132443). sh ATG5 (TRCN0000151963) was purchased from Sigma-Aldrich. .. Wild-type MTFP1 inserts cloned into pGenLenti from Genscript were previously reported [ ]. si PPARGC1A/PGC1α (sc -38,884) and scrambled siRNA (siControl; sc -37,007) were obtained from Santa Cruz Biotechnology.

    Article Title: PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy.
    Article Snippet: Autophagy ISSN: 1554-8627 (Print) 1554-8635 (Online) Journal homepage: www.tandfonline.com/journals/kaup20 PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy Soumya Ranjan Mishra , Priyadarshni Mishra , Kewal Kumar Mahapatra , Bishnu Prasad Behera , Gajanan Kendre , Moureq Rashed Alotaibi , Vijay Pandey , Birija Sankar Patro , Daniel J Klionsky & Sujit Kumar Bhutia To cite this article: Soumya Ranjan Mishra , Priyadarshni Mishra , Kewal Kumar Mahapatra , Bishnu Prasad Behera , Gajanan Kendre , Moureq Rashed Alotaibi , Vijay Pandey , Birija Sankar Patro , Daniel J Klionsky & Sujit Kumar Bhutia (27 Aug 2025): PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy, Autophagy, DOI: 10.1080/15548627.2025.2552900 To link to this article: https://doi.org/10.1080/15548627.2025.2552900 © 2025 The Author(s).. Published by Informa UK Limited, trading as Taylor & Francis Group.. View supplementary material Accepted author version posted online: 27 Aug 2025.

    Clone Assay:

    Article Title: LMNA R644C Associates With Hepatic Steatosis in a Large Cohort and Increases Cellular Lipid Droplet Accumulation in Vitro
    Article Snippet: .. The LMNA open reading frame was cloned into mCherry-Parkin, Addgene plasmid #23956 (replacing the Parkin open reading frame), via the BspEI and BamHI sites. mCherry-Parkin was a gift from Richard Youle (Addgene plasmid #23956; http://n2t.net/addgene:23956; RRID:Addgene 23956)15. .. The R644C variant of LMNA was generated using the QuikChange II site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) and verified via Sanger sequencing.

    other:

    Article Title: The impact of HBx protein on mitochondrial dynamics and associated signaling pathways strongly depends on the hepatitis B virus genotype
    Article Snippet: The NF-кB luciferase reporter plasmid was purchased from GenScript.

    Control:

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression
    Article Snippet: siRNAs against BAX (#2: HSS141355 and #3: HSS141356, Thermo Fisher Scientific) and p53 (TP53, #2: HSS186390 and #3: HSS186391, Thermo Fisher Scientific) or AllStars Negative Control siRNA (QIAGEN, Venlo, The Netherlands) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. .. The plasmid hBax C3-EGFP, a gift from Richard Youle (pEGFP-C3-hBAX: Addgene plasmid #19741; https://www.addgene.org/19741/ (accessed on 11 August 2025); RRID:Addgene_19741; Addgene, Watertown, MA, USA) [ ], and the control plasmid pEGFP-C3 (GenBank accession # U57607 ; Takara Bio Inc., Shiga, Japan) were transiently transfected using Lipofectamine 2000 (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. ..

    Transfection:

    Article Title: HDAC Class I Inhibitor Domatinostat Induces Apoptosis Preferentially in Glioma Stem Cells Through p53-Dependent and -Independent Activation of BAX Expression
    Article Snippet: siRNAs against BAX (#2: HSS141355 and #3: HSS141356, Thermo Fisher Scientific) and p53 (TP53, #2: HSS186390 and #3: HSS186391, Thermo Fisher Scientific) or AllStars Negative Control siRNA (QIAGEN, Venlo, The Netherlands) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. .. The plasmid hBax C3-EGFP, a gift from Richard Youle (pEGFP-C3-hBAX: Addgene plasmid #19741; https://www.addgene.org/19741/ (accessed on 11 August 2025); RRID:Addgene_19741; Addgene, Watertown, MA, USA) [ ], and the control plasmid pEGFP-C3 (GenBank accession # U57607 ; Takara Bio Inc., Shiga, Japan) were transiently transfected using Lipofectamine 2000 (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions. ..

    Cloning:

    Article Title: PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy
    Article Snippet: PPA2 polyclonal antibody (PA5–56816, 1:10000), V5 Tag polyclonal antibody (A190-119A), Alexa Fluor 568 (A-11004; anti-mouse, 1:500), Alexa Fluor 568 (A-11011; anti-rabbit, 1:500), Alexa Fluor 488 (A-11008; anti-rabbit, 1:500), and Alexa Fluor 488 (A-11001; anti-mouse, 1:500) were purchased from Invitrogen. .. PPA2 (HsCD00950939) plasmid was purchased from DNASU Plasmid Repository. pHAGE-mt-mKeima was a gift from Richard Youle (Addgene 131626), pLKO.1 - TRC cloning vector was a gift from David Root (Addgene 10878), psPAX2 was gifted by Didier Trono (Addgene 12260), pMD2.G was gifted by Didier Trono (Addgene 12259), pTFAM.1.0-gDNA was a gift from Kevin White (Addgene 132443). sh ATG5 (TRCN0000151963) was purchased from Sigma-Aldrich. .. Wild-type MTFP1 inserts cloned into pGenLenti from Genscript were previously reported [ ]. si PPARGC1A/PGC1α (sc -38,884) and scrambled siRNA (siControl; sc -37,007) were obtained from Santa Cruz Biotechnology.

    Article Title: PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy.
    Article Snippet: Autophagy ISSN: 1554-8627 (Print) 1554-8635 (Online) Journal homepage: www.tandfonline.com/journals/kaup20 PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy Soumya Ranjan Mishra , Priyadarshni Mishra , Kewal Kumar Mahapatra , Bishnu Prasad Behera , Gajanan Kendre , Moureq Rashed Alotaibi , Vijay Pandey , Birija Sankar Patro , Daniel J Klionsky & Sujit Kumar Bhutia To cite this article: Soumya Ranjan Mishra , Priyadarshni Mishra , Kewal Kumar Mahapatra , Bishnu Prasad Behera , Gajanan Kendre , Moureq Rashed Alotaibi , Vijay Pandey , Birija Sankar Patro , Daniel J Klionsky & Sujit Kumar Bhutia (27 Aug 2025): PPA2 activates MTFP1-DNM1L fission signaling to govern mitochondrial proliferation and mitophagy, Autophagy, DOI: 10.1080/15548627.2025.2552900 To link to this article: https://doi.org/10.1080/15548627.2025.2552900 © 2025 The Author(s).. Published by Informa UK Limited, trading as Taylor & Francis Group.. View supplementary material Accepted author version posted online: 27 Aug 2025.



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